University of Pittsburgh

Li Lan, MD

Assistant Professor





	
	


			

		Dr. Lan	
		

		

	
Contact

    		

		lanl@upmc.edu	
		

		
			

		412-623-3276	
		

		
	
	
			

		Fax: 
		412-623-7761	
		

	    

    
    
   
    
   
    
    		

		
2.6 Hillman Cancer Center
5117 Centre Avenue
	
		

	    
    
   
   
   
   
   
   
    
    
	
	
	
			

		Pittsburgh, PA 15213-1862	
		

		
		
	



	
Education
	
	

	
MD, Tohuku University, Sendai (Japan)
        

              
   
  
 
 	
Research Summary
	
	

	
My major
research field is in DNA damage and repair. We developed several novel methods
for producing various type of DNA damage in situ including using laser combined
with confocal microscope. By using these novel methods, we successfully
revealed the dynamic damage response of DNA repair proteins to DNA damage in
cells. We are also interested in studying how chromatin remodeling and histone
modifications affect each distinct repair pathway, such as repair of
single-strand breaks (SSB), double-strand breaks (DSB) and nucleotide excision
repair (NER). We found specific ATPase dependent chromatin remodeling factors,
ACF and SNF2H, which are necessary for repair of DSB; we established an in vitro system to show ubiquitination
of histone H2A destabilizes nucleosome and facilitates the initiation of NER. Our goal is to determine
the specific chromatin remodeling factors, which are necessary for distinct
repair pathway by siRNA screening, we will study the mechanisms using in vivo
and in vitro approaches. This
study will greatly advance our understanding of mechanisms of maintenance of
genome instability and would be extremely useful for understanding mechanisms
for neurodegenerative diseases and establishing novel targets for cancer
chemotherapy.
	
	




  
 
 
 
 
 
 
 
         
        
        
        
        
        
        
        
        
 
 
   
   
















        









	
Research Lab Affiliation
	
	

	Rapic-Otrin Lab	
	













	
Publications
	
	

	
Lan, L; Nakajima, S; Kapetanaki, M. G; Hsieh, C. L; Fagerburg, M; Thickman, K; Rodriguez-Collazo, P; Leuba, S. H; Levine, A. S; and Rapic-Otrin, V. (2012) Monoubiquitinated histone H2A destabilizes photolesion-containing nucleosomes with concomitant release of UV-damaged DNA-binding protein E3 ligase. J Biol Chem. 287: 12036-12049. |  View Abstract
Lan, L; Ui, A; Nakajima, S; Hatakeyama, K; Hoshi, M; Watanabe, R; Janicki, S. M; Ogiwara, H; Kohno, T; Kanno, S; and Yasui, A. (2010) The ACF1 complex is required for DNA double-strand break repair in human cells. Mol Cell. 40: 976-987. |  View Abstract
Wei, L; Lan, L; Hong, Z; Yasui, A; Ishioka, C; and Chiba, N. (2008) Rapid recruitment of BRCA1 to DNA double-strand breaks is dependent on its association with Ku80. Mol Cell Biol. 28: 7380-7393. |  View Abstract
Kamath-Loeb, A. S; Lan, L; Nakajima, S; Yasui, A; and Loeb, L. A. (2007) Werner syndrome protein interacts functionally with translesion DNA polymerases. Proc Natl Acad Sci USA. 104: 10394-10399. |  View Abstract
Yoshimura, M; Kohzaki, M; Nakamura, J; Asagoshi, K; Sonoda, E; Hou, E; Prasad, R; Wilson, S. H; Tano, K; Yasui, A; Lan, L; Seki, M; Wood, R. D; Arakawa, H; Buerstedde, J. M; Hochegger, H; Okada, T; Hiraoka, M; and Takeda, S. (2006) Vertebrate POLQ and POLbeta cooperate in base excision repair of oxidative DNA damage. Mol Cell. 24: 115-125. |  View Abstract
Lan, L; Nakajima, S; Komatsu, K; Nussenzweig, A; Shimamoto, A; Oshima, J; and Yasui, A. (2005) Accumulation of Werner protein at DNA double-strand breaks in human cells. J Cell Sci. 118: 4153-4162. |  View Abstract
Lan, L; Nakajima, S; Oohata, Y; Takao, M; Okano, S; Masutani, M; Wilson, S. H; and Yasui, A. (2004) In situ analysis of repair processes for oxidative DNA damage in mammalian cells. Proc Natl Acad Sci USA. 101: 13738-13743. |  View Abstract