Satoshi Nakajima, PhD

Assistant Professor


Dr. Nakajima

Contact

412-623-3276
Fax: 412-623-7763

2.6 Hillman Cancer Center

5117 Centre Avenue

Pittsburgh, PA 15213-1862

Education

PhD in Molecular Biology, Tohoku University, Sendai (Japan)

Research Summary

Genome stability is maintained by a complicated network including DNA repair, DNA replication, transcription, cell cycle checkpoint, chromatin remodeling, and apoptosis. These pathways do not work independently but closely cooperate with each other. It is difficult to explain function and relationship of the network entirely by existing known factors. The current focus of Dr. Nakajima’s research is to identify a novel factor by a unique screening method to clarify the complicated network. Using biological, genetics, proteomics, and biochemical techniques, Dr. Nakajima will investigate functions and significance of identified genes. Potential of the characterized proteins as a diagnosis tool and an alternative therapy for the radiation resistance cancer will be expected.

Research Lab Affiliation

Publications

Lan, L; Nakajima, S; Wei, L; Sun, L; Hsieh, C.L; Sobol, R.W; Bruchez, M; Van Houten, B; Yasui, A and Levine, A.S. (2013) Novel method for site-specific induction of oxidative DNA damage reveals differences in recruitment of repair proteins to heterochromatin and euchromatin. Nucleic Acids Res. [Epub ahead of print] |  View Abstract

Lan, L; Nakajima, S; Kapetanaki, M. G; Hsieh, C. L; Fagerburg, M; Thickman, K; Rodriguez-Collazo, P; Leuba, S. H; Levine, A. S; and Rapic-Otrin, V. (2012) Monoubiquitinated histone H2A destabilizes photolesion-containing nucleosomes with concomitant release of UV-damaged DNA-binding protein E3 ligase. J Biol Chem. 287: 12036-12049. |  View Abstract

Lan, L; Ui, A; Nakajima, S; Hatakeyama, K; Hoshi, M; Watanabe, R; Janicki, S. M; Ogiwara, H; Kohno, T; Kanno, S; and Yasui, A. (2010) The ACF1 complex is required for DNA double-strand break repair in human cells. Mol Cell. 40: 976-987. |  View Abstract

Nakajima, S; Lan, L; Kanno, S; Usami, N; Kobayashi, K; Mori, M; Shiomi, T; and Yasui, A. (2006) Replication-dependent and -independent responses of RAD18 to DNA damage in human cells. J Biol Chem. 281: 34687-34695. |  View Abstract

Lan, L; Nakajima, S; Komatsu, K; Nussenzweig, A; Shimamoto, A; Oshima, J; and Yasui, A. (2005) Accumulation of Werner protein at DNA double-strand breaks in human cells. J Cell Sci. 118: 4153-4162. |  View Abstract

Nakajima, S; Lan, L; Kanno, S; Takao, M; Yamamoto, K; Eker, A. P; and Yasui, A. (2004) UV light-induced DNA damage and tolerance for the survival of nucleotide excision repair-deficient human cells. J Biol Chem. 279: 46674-46677. |  View Abstract

Lan, L; Nakajima, S; Oohata, Y; Takao, M; Okano, S; Masutani, M; Wilson, S. H; and Yasui, A. (2004) In situ analysis of repair processes for oxidative DNA damage in mammalian cells. Proc Natl Acad Sci USA. 101: 13738-13743. |  View Abstract